Age-related changes in isocitrate lyase from the free living nematode, Turbatrix aceti.
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چکیده
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Isocitrate lyase from Phycomyces blakesleeanus
Isocitrate lyase was purified from Phycomyces blakesleeanus N.R.R.L. 1555(-). The native enzyme has an Mr of 240000. The enzyme appeared to be a tetramer with apparently identical subunits of Mr 62000. The enzyme requires Mg2+ for activity, and the data suggest that the Mg2+-isocitrate complex is the true substrate and that Mg2+ ions act as a nonessential activator. The kinetic mechanism of the...
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3-Phosphoglycerate kinase from Turbatrix aceti, when partially pure, shows a molecular weight which varies from 43,000 to 160,000 on columns of Sephadex G-150 or G-10~ depending upon the eluting buffer and its ionic strength. The pure enzyme has an unchanging molecular weight of 43,000 ± 2,000. Similar shifts of molecular weight have been observed in partially purified preparations of phosphogl...
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Biogenic amines serve as neurotransmitters in vertebrates and insects, and their occurrence has been reported in a number of other species of invertebrates. Among helminths, they have been isolated from the liver fluke Fasciola hepatica (9) and the nematodes Ascar# lumbricoides (10), Ascaridia galli (10), Nippostrongylus brasiliensis (1), Litomosoides carinii (12), Caenorhabditis elegans (8), a...
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Escherichia coli isocitrate lyase (EC 4.1.3.1.) can be phosphorylated in vitro by an ATP-dependent reaction. The enzyme becomes phosphorylated by an endogenous kinase when partially purified sonic extracts are incubated with [gamma-32P]ATP. Treatment of isocitrate lyase with diethyl pyrocarbonate, a histidine-modifying reagent, blocked incorporation of [32P]phosphate from [gamma-32P]ATP. The is...
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The Lo Go-Xa Mat is a national park in the southeastern region of Vietnam, which has a particularly high biodiversity and it includes different wetlands which are unique diverse in species composition. It can be categorized into two types: temporarily-seasonally and permanently flooded wetlands. Ta Not grassy marsh is representative of the seasonally flooded wetland. Whilst the diversity and ec...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1975
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)41859-0